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cytotec online purchase in singapore Twenty four hours after transfection the media was replaced with fresh phenol red free media supplemented with 5 charcoal stripped FBS v v and the cells were treated with vehicle ethanol, E 2 1 nM or OH Tam 100 nM for the following five days; the culture media was not changed during this period but E 2 and OH Tam were replenished every 48 h

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